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Baculo Virus

Baculovirus Expression

  • Transfection of insect cells and generation of recombinant virus
  • Viral production and plaque purification
  • Test expression of recombinant virus clones
  • Amplification and purification of recombinant baculovirus
  • Viral titer determination and generation of high-titer virus stock
  • Expression optimization is Sf9 or HiFive cells and varying temperature, MOI, growth conditions, media
  • Solubility analysis
  • Large-scale culture protein production/li>
  • Cell harvesting (or conditioned medium) and lvsate preparation
  • High throughput screening of your multiple plasmids to find the most desired construct
  • Producing high titer viruses in one week