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E. Coli

E. Coli Expression


  • Transformation of the expression vector into E.Coli
  • Screening and selection of positive clones
  • Evaluation of expression by SDS-PAGE or Western blotting
  • Optimization of host strain with small-scale analysis in different E.Coli backgrounds
  • Growth and expression optimization by varying induction, temperature, cell density, time, antibiotics, media to overcome low production and/or insoluble protein production
  • Inclusion body isolation and preparation
  • Cell harvesting and lvsate preparation